Detection and Characterization of a Multi-drug and Multi-metal Resistant Enterobacterium Pantoea sp. from Tannery Wastewater after Secondary Treatment Process

R.N. Bharagava, Abhay Raj, Naresh Bharagava

Abstract


In this study, an enterobacterium was isolated from treated tannery wastewater and
characterized as gram-negative, rod shaped, motile, and lactose fermenting bacterium.
Further, based on the 16S rRNA gene sequence analysis, the bacterium was identified as
Pantoea sp. with accession number KJ576899. The antibiotic and heavy metal resistant
property of isolated bacterium was investigated by the disk diffusion method on Muller-
Hinton and nutrient agar medium amended with the increasing concentrations of
various toxic metal ions, respectively. Results showed that the isolated bacterium was
sensitive for amikacin, ampicillin, cefepime, cetriaxone, chloramphenicol, levofloxacin,
meropenem, nalidixic acid, piperacillin and tobramycin, resistant for aztreonam,
carbenicillin, cefotaxime, ceftazidime, ciprofloxacin, cotrimoxazole, imepenam,
moxifloxacin, streptomycin and tetracycline, but intermediate for amoxicillin and
gentamicin. In addition, the bacterium also showed the Minimum Inhibitory
Concentration (MIC) values of 250, 500, 160, 190, 600, 700, 500, 380, 600 and 350 μg ml-1
for Cu, Cr, Cd, Co, Zn, Fe, Ni, Pb, Mo and As, respectively with a plasmid DNA of 33.5 kb. This
multi-drug and multi-metal resistant bacterium can be used as a potential agent for the
bioremediation of metal contaminated sites.

Keywords


Antibiotic Enterobacterium, Metal resistant, Minimum Inhibitory Concentration, Pantoea sp., Tannery wastewater, Toxic metals

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DOI: http://dx.doi.org/10.18811/ijpen.v2i1-2.6616

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