Detection of VP6 gene of Rotavirus in Feces of Diarrhoeic calves, kids, lambs, piglets, pups and human infants by Reverse Transcriptase–Polymerase Chain Reaction

U. M. Tumlam, V. C. Ingle, P. A. Tembhurne, N. V. Kurkure, S. P. Chaudhari, S. D. Chitambar, S. Bhoyar

Abstract



The present study was undertaken VP6 gene based detection of Rotavirus in faeces of diarrheic bovine, porcine, caprine, ovine, canine species of animals and human. A total of 280 faecal samples from bovine calves, piglets, kids, lambs, pups and human infants (0-1 yr of age) were screened for the detection of group A Rotavirus by VP6 gene based RT–PCR assay. A Total of 43 (15.35%) samples were found positive for Rotavirus. Maximum 17/50 (34.00%) samples were positive in human infants followed by 7/71 piglets (9.85%), 7/25 lambs (28.00%), 6/26 kids (23.07%), 4/58 bovine calves (6.89%) and 2/50 pups (4.00%).
VP6 gene base RT-PCR study confirmed the prevalence of group A Rotavirus in animals and human species. RT–PCR assay can be used as a sensitive and specific assay for the rapid detection of group A Rotavirus in faecal samples.

Keywords


Group A Rotavirus, VP6 gene, diarrhoea, RT–PCR

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DOI: https://doi.org/10.21887/.v13i04.11551

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