Isolation of a new flavanone and evaluation of antidiabetic activity from Clematis buchananiana aerial parts

U. P. Bhatt; Sudhir Kumar; Bipin Rawat; Subhash Chandra; Sarla Saklani; R. P. Bahuguna

U. P. Bhatt Government Polytechnic Gopeshwar, Chamoli, Uttarakhand, India.
Sudhir Kumar Department of Chemistry, Govt. PG College, Gopeshwar, Chamoli, Uttarakhand, India.
Bipin Rawat Department of Chemistry, H. N. B. Garhwal University (A Central University), Srinagar-246174, Uttarakhand, India.
Subhash Chandra Department of Pharmaceutical Chemistry, H. N. B. Garhwal University (A Central University), Srinagar-246174, Uttarakhand, India.
Sarla Saklani Department of Pharmaceutical Chemistry, H. N. B. Garhwal University (A Central University), Srinagar-246174, Uttarakhand, India.
R. P. Bahuguna Department of Chemistry, H. N. B. Garhwal University (A Central University), Srinagar-246174, Uttarakhand, India.

Keywords: Alloxan Blood glucose Clematis buchananiana Diabetes mellitus α-Amylase

Abstract

The present study aimed to explore the phytochemistry and antidiabetic activity of Clematis buchananiana DC. A new compound (1) characterized as 3-[(11’’-hydroxy, 3’’,6’’,10’’,10’’tetramethyldodecene)] 6-[(3’’’,4’’’,4’’’-trimethylhexene)] 5,7,2’,4’,6’pentahydroxy 8, 3’,5’ tri methyl flavanone was isolated from the aerial parts of the plant. The methanolic extract of the plant at a concentration of 1 mg/mL showed a 75.00% reduction in α-amylase and 73.13% in αglucosidase enzyme activity. Moreover, the methanolic extract at 100 mg/kg b.w. showed a significant decline in blood glucose levels of alloxan-induced diabetic rats both in acute and chronic studies. The results were compared with glibenclamide, an oral hypoglycemic agent, which was used as a positive control. The present study concludes that the plant has substantial antidiabetic potential in diabetic rats. These results are indicative and further studies are needed to revalidate the findings.