Molecular detection and antibiogram of Methicillin resistant Staphylococcus aureus isolated from the nares of cattle in Aizawl, Mizoram

Lalnunfela .; Blessa Sailo; C Lalremruata; Joy Lalmuanpuia

doi:10.56678/iahf-2023.36.02.3

Lalnunfela . Dept. of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and Animal Husbandry, CAU (I), Selesih, Mizoram-796015
Blessa Sailo ICAR- Manipur Centre, Lamphelpat, Imphal, Manipur-795004
C Lalremruata KVK, North Vanlaiphai, Serchhip district, Mizoram-796184
Joy Lalmuanpuia Dept. of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and Animal Husbandry, CAU (I), Selesih, Mizoram-796015

DOI: https://doi.org/10.56678/iahf-2023.36.02.3

Keywords: MRSA, Prevalence, Staphylococcus aureus

Abstract

A total of 160 nasal swabs were collected from cattle and processed for Staphylococcus aureus isolation. All samples were initially screened for the presence of S. aureus. All the isolates were confirmed by PCR using a species-specific primer (nuc gene), yielding an overall prevalence rate of 8.125%. The positive isolates were then screened for MRSA targeting the mecA gene, which had a 5.0% prevalence rate. The study's findings on MRSA's antimicrobial sensitivity pattern indicate a significant prevalence of resistance to oxacillin and ampicillin.