Identification of sex linked molecular markers in spine gourd (Momordica dioica Roxb.)

Gajala . Ameen; Jitendra Kumar Tiwari; Ved . Prakash; Vivek Kumar Sandilya; B. K. Das

doi:10.31742/IJGPB.82.1.17

Gajala . Ameen Section of Genetics & Plant Breeding, Raj Mohini Devi College of Agriculture and Research Station, Indira Gandhi Krishi Vishwavidyalaya, Ambikapur 497 001, Chhattisgarh, India
Jitendra Kumar Tiwari Section of Genetics & Plant Breeding, Raj Mohini Devi College of Agriculture and Research Station, Indira Gandhi Krishi Vishwavidyalaya, Ambikapur 497 001, Chhattisgarh, India
Ved . Prakash Section of Genetics & Plant Breeding, Raj Mohini Devi College of Agriculture and Research Station, Indira Gandhi Krishi Vishwavidyalaya, Ambikapur 497 001, Chhattisgarh, India
Vivek Kumar Sandilya Section of Genetics & Plant Breeding, Raj Mohini Devi College of Agriculture and Research Station, Indira Gandhi Krishi Vishwavidyalaya, Ambikapur 497 001, Chhattisgarh, India
B. K. Das Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, DAE, Mumbai 400 085

DOI: https://doi.org/10.31742/IJGPB.82.1.17

Keywords: DNA extraction, Momordica dioica, PCR amplification, simple sequence repeat (SSR)

Abstract

The major drawback of a dioecious spine gourd (Momordica dioica Roxb.) is distinguishing whether the plant is male or female at an earlier development stage. An investigation was aimed to eliminate aforesaid limitation by identifying and validating sex linked molecular marker. Twenty six male and female genotypes of each sex were screened with 12 specific SSR primers. Only two SSR primers, MdSSR 5 (MADS-box gene of spine gourd) and CMbSSR 6 (MADS-box gene of cucumber) showed amplification and polymorphism in female genotypes only. These specific female linked markers manifest existent of non-epigenetic factors entailed in gender development in the spine gourd.