Characterization of novel soybean derived simple sequence repeat markers and their transferability in hyacinth bean [Lablab purpureus (L.) Sweet]

L. M. Yao; L. D. Zhang; Y. L. Hu; B. Wang; T. L. Wu

L. M. Yao Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai China
L. D. Zhang Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai China
Y. L. Hu Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai China
B. Wang Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai China
T. L. Wu Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai China

Keywords: Soybean; Lablab purpureus; EST-SSR; transferability

Abstract

Simple Sequence Repeat (SSR) markers were developed from soybean expressed sequence tag (EST) sequences obtained from The European Molecular Biology Laboratory (EMBL) database. Of the 1517 loci considered 764 (50.4%) and 573 (37.8%) constituted tri and dinucleotide and, 73 (4.8%), 44 (2.9%), 38 (2.5%) and 25 (1.6%) represented mono, hexa, tetra and pentanucleotide repeats, respectively. Fifty of these SSR makers exhibited 100% transferability in Lablab purpureus. Eight out of 50 loci (16%) displayed length polymorphisms. BLAST results showed that the function of only 15 EST sequences (30%) of the selected 50 SSR sequences were known. The novel EST-SSR will be useful for developing new molecular markers of L. purpureus.