An improved cytogenetic technique intended for macroalgal chromosomes

Sandeep Dhankhar; C. R. K. Reddy; B. Jha; Seema Dhankhar

Sandeep Dhankhar Discipline of Marine Chemicals Research Institute, Council of Scientific and Industrial Res., Bhavnagar 364 021
C. R. K. Reddy Discipline of Marine Chemicals Research Institute, Council of Scientific and Industrial Res., Bhavnagar 364 021
B. Jha Discipline of Marine Chemicals Research Institute, Council of Scientific and Industrial Res., Bhavnagar 364 021
Seema Dhankhar Discipline of Marine Chemicals Research Institute, Council of Scientific and Industrial Res., Bhavnagar 364 021

Keywords: Chromosome, chlorophyta, rhodophyta, cytogenetic, macroalgae

Abstract

The selection of fixative was exercised Therefore, Different fixatives such as acetic acid, acetone, chloroform, methanol, ethanol and formaldehyde were used separately or in combination in altered ratios to optimize it for chromosome staining in macroalgae (seaweeds). Combination of acetic acid with formaldehyde (1:1) was found to be effective for chromosomes staining in Chlorophyta and Rhodophyta. Moreover, the corbol fuchsins and aceto-iron–haematoxylin-chloral-hydrate were found to be effective stains for Rhodophyta and Chlorophyta respectively. These techniques were used to prepare karyological studies in seaweeds including seven Ulva spp., Gracilaria spp., Monostroma spp. and a few Porphyra species.