Development of EST-derived microsatellite markers in mungbean [Vigna radiata (L.) Wilczek] and their transferability to other Vigna species

Abstract

Twenty microsatellite markers were developed from mungbean [Vigna radiata (L.) Wilczek] EST sequences available in public database. Out of these, ten microsatellite markers (50%) were found to be polymorphic when screened on 22 genotypes. The number of alleles ranged from two to three with an average of 2.3 alleles per locus. Polymorphic information content of the microsatellite markers ranged from 0.09 to 0.59 with an average of 0.31. When these microsatellite markers were screened on eight other Vigna species, they showed high transferability (90%) to other Vigna species. These ESTderived microsatellite markers may be useful for linkage mapping, gene tagging and genetic diversity analysis in mungbean and other related Vigna species.