Development and characterization of microsatellite markers for sour jujube (Ziziphus jujuba var. spinosa)

Chunmei Zhang; Xiao Yin; Xingang Li; Jian Huang; Changzhu Wang; Chunlan Lian

Chunmei Zhang College of Forestry, Northwest AandF University, Yangling, Shaanxi 712100, China
Xiao Yin College of Forestry, Northwest AandF University, Yangling, Shaanxi 712100, China
Xingang Li College of Forestry, Northwest AandF University, Yangling, Shaanxi 712100, China
Jian Huang College of Forestry, Northwest AandF University, Yangling, Shaanxi 712100, China
Changzhu Wang College of Forestry, Northwest AandF University, Yangling, Shaanxi 712100, China
Chunlan Lian College of Forestry, Northwest AandF University, Yangling, Shaanxi 712100, China

Keywords: Sour jujube; Ziziphus jujuba var. spinosa; SSR markers; genetic diversity; population genetics

Abstract

Sour jujube (Ziziphus jujuba var. spinosa) is an important wild fruit tree and traditional Chinese medicine. Ten microsatellite markers of sour jujube were developed using a modified dual-suppression-PCR technique. Polymorphism of each locus was detected in 40 individuals from three natural populations.The number of alleles per locus ranged from 2 to 10, with an average of 6.4. The expected and observed heterozygosities ranged from 0.420 to 0.832 and 0.161 to 0.975, respectively. The markers will be useful for future genetic diversity and population genetic studies, and may help to establish strategies for conservation of this species.