Molecular marker assisted back cross breeding for effective transfer of Lr19 in wheat (Triticum aestivum L.)

Dharam Pal; S. C. Bhardwaj; Pratima Sharma; Deepak Sharma; Shilpi Kumari; Madhu Patial; K. V. Prabhu; J. Kumar

Dharam Pal School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan
S. C. Bhardwaj ICAR-Central Arid Zone Research Institute, Jodhpur 342 003
Pratima Sharma Department of Botany, Government College University, Faisalabad
Deepak Sharma Botany Department, University of the Punjab, Lahore 54590, Pakistan
Shilpi Kumari Botany Department, University of the Punjab, Lahore 54590, Pakistan
Madhu Patial Botany Department, University of the Punjab, Lahore 54590, Pakistan
K. V. Prabhu Botany Department, University of the Punjab, Lahore 54590, Pakistan
J. Kumar Botany Department, University of the Punjab, Lahore 54590, Pakistan

Keywords: Wheat, SCAR marker, Lr19, leaf rust

Abstract

The gene Lr19, derived from Agropyron elongatum provides effective resistance against leaf rust in Northern India. To develop improved wheat lines in the background of HS240, HS295, rust resistance from FLW20 possessing Lr19 was involved to generate back cross populations and confirm resistance through host-pathogen interaction and molecular marker. The SCAR marker SCS265512 was used to validate Lr19 in back cross plants transferred from FLW20. Results of HPI test and molecular marker assisted validation for Lr19 showed 88-93 percent consistency, indicating that both the techniques are mutual exclusive for effective selection of Lr19. Fixed gene populations are now ready for station trials.