Molecular mapping of leaf rust resistance gene in wheat line 19HRWSN-76

Han Liusha; Li Zaifeng; Wang Jiazhen; Shi Lingzhi; Zhu Lin; Li Xing; Liu Daqun

Han Liusha College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001
Li Zaifeng College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001
Wang Jiazhen College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001
Shi Lingzhi College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001
Zhu Lin College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001
Li Xing College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001
Liu Daqun College of Plant Protection, Biological Control Centre of Plant Diseases and Plant Pests of Hebei, Agricultural University of Hebei, Baoding, China 071 001

Keywords: Leaf rust resistance gene, molecular marker, genetic analysis, molecular mapping

Abstract

A highly leaf rust resistant genetic stock 19HRWSN-76 from CIMMYT was crossed to a susceptible variety Zhengzhou 5389 to generate F1 and F2 population to find out the mode of inheritance and map the resistance gene. The F2 population was inoculated with uredosporic inoculum of physiological race FHJP of leaf rust. The 3:1 ratio of resistant and susceptible plants in F2 suggested that the leaf rust resistance in 19HRWSN-76 is controlled by a single gene, tentatively named Lr19HRWSN-76. Relationship between the known leaf rust resistance gene Lr24 and Lr19HRWSN- 76 located on chromosome 3D was established by SSR marker barc71 and STS 24-16.