An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen

P. Jayaprakash; S. Annapoorani; V. K. Vikas; M. Sivasamy; J. Kumar; K. Saravannan; E. Punniakotti; D. Sheeba

P. Jayaprakash ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
S. Annapoorani ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
V. K. Vikas ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
M. Sivasamy ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
J. Kumar ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
K. Saravannan ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
E. Punniakotti ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
D. Sheeba ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu

Keywords: Recalcitrant wheat pollen, peptone water, maltose, e-amino caproic acid (EACA), in vitro pollen germination

Abstract

There is no robust and quick technique available for testing germination of wheat pollen. In this study, Brewbaker and Kwack (BK) pollen germination medium (PGM) was adjusted with a-amino caproic acid (EACA), peptone water and maltose to develop an improved medium for wheat. Germination of pollen in this PGM ranged from 92% to 98.2% depending upon genotype pollen germination media (PGM). This is the first report of PGM which has >95% wheat pollen germination in vitro.