Assessment of double haploid culture conditions in bell pepper (Capsicum annuum L.)

Anjanabha Bhattacharya; Bhavesh Palan; Kishor Mali; Bharat Char

Anjanabha Bhattacharya Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
Bhavesh Palan Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
Kishor Mali Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
Bharat Char Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra

Keywords: Bell Pepper, double haploid, embryoids, Murashige and Skoog media, plantlet regeneration

Abstract

The present study was performed to generate double haploid lines in bell pepper with high regeneration efficiency via anther culture. In total, 825 anthers were plated on five different modified MS media viz., MS I, MS II, MS III, MS IV and MS V. The number of embryoids formed was found to be highest on MS IV media (26 embryoids) as compared to others. Forty five embryoids were developed on five media combinations, out of which 19 were regenerated into plantlets. Therefore, the optimized media i.e. MS IV in this study may be suitable for the development of double haploid lines in other economically important bell pepper as well as hot pepper genotypes through anther culture.