A molecular marker linked to the male gender of Actinidia arguta Siebold and Zucci

X. Z. Liu; T. W. Lv; H. M. Liu; H. Y. Zhang

X. Z. Liu Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, 650224 Yunnan Province, People’s Republic of China
T. W. Lv Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, 650224 Yunnan Province, People’s Republic of China
H. M. Liu Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, 650224 Yunnan Province, People’s Republic of China
H. Y. Zhang Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, 650224 Yunnan Province, People’s Republic of China

Keywords: Actinidia arguta, gender identification, RAMP, SCAR

Abstract

To distinguish the female plants in early stage, BSA and RAMP techniques were employed to screen and characterize molecular markers linked to hardy kiwi gender. One RAMP marker GA(CT)4/S129450, was found to be tightly linked to the male gender. Then the RAMP maker was cloned, sequenced, and conversed into SCAR marker. And early stage selection was carried out using the SCAR marker on 142 F1 progeny from crossing between Yongshan 11 and Yongshan 5. It is the first report on distinguishing the gender of hardy kiwi in early stage.