Analysis of molecular diversity in Indian and Exotic genotypes of Brassica juncea using SSR markers

  • Jebi Sudan School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180 001 (J and K)
  • Prakriti Khajuria School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180 001 (J and K)
  • S. K. Gupta Department of Plant Breeding and Genetics, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180 001 (J and K)
  • Ravinder Singh School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180 001 (J and K)
Keywords: Brassica juncea, genetic diversity, population structure, dendrogram

Abstract

Molecular diversity analysis of Indian and Exotic genotypes of Brassica juncea genotypes was carried out using simple sequence repeat (SSR) markers. Sixteen out of 32 SSR markers were found to be polymorphic and amplified 54 alleles in 23 genotypes with an average of 2.37 and 0.31 for alleles per locus and polymorphic information content (PIC), respectively. The genotypes were grouped into three distinct clusters based on unweighted neighbour joining and population structure analyses. The molecular analysis revealed that markers obtained from diploid progenitors were useful for molecular diversity studies in Brasicca juncea.
Published
2016-08-25