Confirmation of some SCAR molecular markers linked to Rhizomania resistance gene (Rz1) in sugar beet

Peyman Norouzi; Seyed Bagher Mahmoudi; Saeed Darabi; Mozhdeh Kakueinezhad

Peyman Norouzi Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Seyed Bagher Mahmoudi Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Saeed Darabi Sugar Beet Research Department, Fars Research Center for Agriculture and Natural Resources, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
Mozhdeh Kakueinezhad Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Keywords: Agreement, breeding, disease, SCAR, selection

Abstract

For the involvement of Rhizomania resistance genes in breeding programs, tagging these genes by molecular markers is necessary. In this study, some breeding populations and commercial varieties of sugar beet originated from Holly source were used for the validation of three repulsion SCAR molecular markers correlated with ELISA and field resistance. Comparison between ELISA and field resistance and molecular markers showed that repulsion marker ZN5 had 96% and 98% agreement with ELISA and field resistance, respectively with 95% and 100% presence in susceptible and resistant varieties, respectively. Therefore, this marker can be used for screening of homozygous lines resistant to rhizomania originated from Rz1 gene source.