Selection of reliable reference genes for gene expression studies by quantitative real-time PCR in Anoectochilus roxburghii

Yanan Zhang; Boyun Yang; Liping Luo; Dongjin Xiong; Xueyong Huang; Huolin Luo

Yanan Zhang Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
Boyun Yang Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
Liping Luo Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
Dongjin Xiong Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
Xueyong Huang Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
Huolin Luo Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China

Keywords: Anoectochilus roxburghii, geNorm, NormFinder, reference gene

Abstract

RT-qPCR is a sensitive, efficient and reliable method for gene expression studies and reference gene expression stability was essential for RT-qPCR. Since previous studies showed that no reference gene could exhibit changeless expression pattern in all experiments, this study determined the gene expression stability of 11 reference genes across various treatments in Anoectochilus roxburghii. The results indicated that expression stability of genes varied considerably under different treatments. The genes, GAPDH, EF1a and ACT1 emerged as the most reliable reference genes.