Development and validation of markers for spike density QTL, Qsd.sau-7A from Tibetan semi-wild wheat (Triticum aestivum ssp. tibetanum)

Jian Ma; Min Sun; Congcong Yang; Nana Qin; Han Zhang; Puyang Ding; Yang Mu; Huaping Tang; Xiujin Lan

Jian Ma Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Min Sun Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Congcong Yang Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Nana Qin Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Han Zhang Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Puyang Ding Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Yang Mu Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Huaping Tang Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
Xiujin Lan Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

Keywords: Spike density, QSd-sau-7A, high resolution melting, validation.

Abstract

Spike density is one of the few traits that determines the yield potential in wheat. Three high resolution melting (HRM) markers (0C32-715, DCE-464, and C81A-240) were developed and used to map the QTL for spike density Qsdsau- 7A. The markers 0C32-715 and DCE-464 were validated in two of the three validation population. DCE-464 and 0C32- 715 were the most closely linked markers that could be used to introgress QSd.sau-7A through molecular maker assisted breeding and development of near isogenic lines.