Inheritance of fertility restoration of A4 cytoplasm in pearl millet [Pennisetum glaucum (L.) R. Br.]

J. Jorben; S. P. Singh; C. Tara Satyavathi; S. Mukesh Sankar; Jayant S. Bhat; Kumar Durgesh; M. Mallik

J. Jorben Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
S. P. Singh Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
C. Tara Satyavathi Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
S. Mukesh Sankar Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
Jayant S. Bhat Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
Kumar Durgesh Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
M. Mallik Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012

Keywords: Inheritance, A4 cytoplasm, fertility restoration, pearl millet

Abstract

Present investigation was carried out to study the mode of inheritance of fertility restoration for A4 cytoplasm using pollen fertility and seed set per cent as criterion in determining the fertile and sterile plants. Two CMS lines of A4 cytoplasm were crossed with two fertility restorers generating four F1 crosses, namely, ICMA 99111 x PPMI 1003, ICMA 99111 x PPMI 1087, ICMA 03999 x PPMI 1003 and ICMA 03999 x PPMI 1087, their F2s and backcross generations. All the F1s were completely fertile indicating complete fertility restoration. F2s and backcross generations were evaluated at IARI, New Delhi and IARI Regional Centre, Dharwad during summer 2017 and χ 2 test was applied to test the significance. At both the locations, all the F2 segregating populations fit well into a Mendelian ratio of 15:1 indicating digenic duplicate dominance of fertility restoring genes with χ 2 value of 0.82, 2.90, 0.04, 3.97, 4.86, 4.98, 0.02, 1.26, 3.15, 4.98, 3.15 and 0.02. The F2 hypothesis was verified with the observed frequency of segregating plants fitting well into 3:1 ration with χ 2 value of 5.45, 1.93, 4.93, 0.60, 2.83, 0.44, 4.94, 2.77, 3.33, 0.13, 4.08 and 1.51. It is further confirmation of the findings that fertility restoration is indeed governed by two duplicate genes. Association between pollen fertility and seed set per cent was significant and positive.