Validation of hybrid rice seed vigour traits using SSR marker (Oryza sativa L.)

Shruti Kumari; S. K. Chakrabarty; P. K. Bhowmick; Vikram Jeet Singh; A. S. Hari Prasad

Shruti Kumari Division of Seed Science and Technology, 1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
S. K. Chakrabarty Division of Seed Science and Technology, 1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
P. K. Bhowmick Division of Seed Science and Technology, 1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
Vikram Jeet Singh Division of Seed Science and Technology, 1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
A. S. Hari Prasad Division of Seed Science and Technology, 1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012

Keywords: Rice, hybrid, seed vigour traits, QTLs, SSR marker

Abstract

Seed vigour is an important characteristic of seed quality. Several quantitative trait locus (QTL) has been reported for seed vigour in many crops. In order to validate seed vigour traits, an experiment was conducted with three released rice hybrids and their respective female (A), and restorer/male lines using SSR markers. A total of 18 quantitative traits loci (QTLs) were chosen for seed vigour characters for which 16 SSR markers that were dispersed throughout the 11 chromosomes of rice have been assessed. Eight SSR markers were polymorphic among the genotypes studied. These markers showed distinct bands in female (A), male (R) and hybrid (H). Further, genuineness of all the hybrids under study could be established using the primer RM 252.