Molecular characterization of Indian Dolichos bean {Lab/ab purpureus L. var. typicus Prain) accessions using RAPD markers

Saidaiah Pidigam; Suchandranathbabu Munnam; Srinivas Nimmarajula; Geetha Amarapalli; Harikishan Sudini; Someshwar Rao Pandravada; Hari Yadla

Saidaiah Pidigam College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana
Suchandranathbabu Munnam College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana
Srinivas Nimmarajula College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana
Geetha Amarapalli College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana
Harikishan Sudini College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana
Someshwar Rao Pandravada College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana
Hari Yadla College of Horticulture, Sri Kanda Laxman Telangana State Horticultural University (SKLTSHU), Rajendranagar 500 030, Hyderabad, Telangana

Keywords: Dolichos bean, genetic diversity, RAPD, STRUCTURE, UPGMA

Abstract

In this study, 29 genotypes of (Lab/ab purpureus) Indian bean augmented from different geographical regions were analyzed for genetic diversity using 39 RAPDs. The binary matrix data revealed 97 .4% polymorphism among genotypes, with 2322 amplification fragments. The PIC values ranged from 0.25 to 0.9 6 with an average of O. 70. Markers OPA05, OPB15, and OPV12 showed high discrimination among all the genotypes. A magnificent range of Jaccard's genetic similarity coefficient was evaluated and UPGMA based dendrogram classified genotypes into 4 major clusters. lC-384066 and NSJ/NAIP/ 192 were found to be unique and highly diversified. The same grouping patterns were also observed in the modelbased STRUCTURE analysis (K=2). The study showed the degree and distribution of genetic diversity in Dolichos bean, which can be utilized to identify the parental lines, to develop mapping populations and breeding.