Diagnosis of HCV Infection Using Different Rapid Methods, ELISA and Anti HCV Test

Varun Kumar Tomer; Sonia .; Bhagat Singh; Mahesh Kumard; Stuti Kaushike; Reena Panwar; Renu Singh

doi:10.54618/IJMAHS.2021117

Varun Kumar Tomer Department of Microbiology J. V. College, Baraut, Baghpat-250 611 Uttar Pradesh, India
Sonia . Department of Microbiology J. V. College, Baraut, Baghpat-250 611 Uttar Pradesh, India
Bhagat Singh Department of Medical Lab. Technology, Faculty of Paramedical Sciences, UPUMS, Saifai, Etawah-206130 India
Mahesh Kumard Department of Microbiology J. V. College, Baraut, Baghpat-250 611 Uttar Pradesh, India
Stuti Kaushike Department of Microbiology J. V. College, Baraut, Baghpat-250 611 Uttar Pradesh, India
Reena Panwar Department of Microbiology J. V. College, Baraut, Baghpat-250 611 Uttar Pradesh, India
Renu Singh Former visiting faculty, Faculty of Paramedical Sciences, UP University of Medical Sciences, Saifai Etawah, 206130 India

DOI: https://doi.org/10.54618/IJMAHS.2021117

Keywords: HCV, Immunochromatography test, ELISA

Abstract

Background: Hepatitis C virus (HCV) mainly cause liver associated problem worldwide. Most of the affected people develop liver cirrhosis which develop as a major health issue. Aim: This study aimed towards HCV infection in rural population in India. Materials and Methods: This study was conducted at Rama Medical College and Research Centre over a period of 6 months from 1 January to 30 June, 2021. A total of 721 patients of age between (1 – 85) years were screened and checked for presence of anti-HCV antibody using ELISA and Immunochromatography test. Result: A total of 53 (7.35%) from 721 samples were positive 26 (49.05%) male and 27 (50.9%) females using Immunochromatography test, and then these 53 positive samples were further tested by ELISA as confirmatory test. Out of 53 samples 48 samples were found true positive while 5 samples were found false positive. Conclusion: HCV Infection in this study was found 6.65% in rural area in Hapur District of Utter Pradesh. Disease mostly observed in elder person (50-65) then younger ones. About 49.05 % males and 50.9 % female were found positive.