Cigarette Smoking Provoked Proinflammatory Cytokines and Oxidative Stress in Healthy Smokers

Hesham A. M. Gomaa; Mohamed F. El Shafie; Khaled Younes Mohamed

Hesham A. M. Gomaa Biochemistry Department, Faculty of Pharmacy, Nahda University, Baniswif, Egypt.
Mohamed F. El Shafie Biochemistry Department, Faculty of Pharmacy, Nahda University, Baniswif, Egypt.
Khaled Younes Mohamed Biochemistry Department, Faculty of Pharmacy, Nahda University, Baniswif, Egypt.

Keywords: Cigarette smoking, smoking index, oxidative stress, inflammation.

Abstract

L-Glutaminase is an anti-leukemic enzyme produced by number of microbes. The three-phase partitioning method of Iso-octane followed by the ammonium sulphate precipitation was used to purify the L-glutaminase produced by Pseudomonas fluorescens. The selected process parameters viz. concentration of ammonium sulphate, Iso-octane to broth ratio, temperature and pH were optimized by Taguchi orthogonal array design of experiment (DOE). Overall, 28.19%, enhanced recovery of L-glutaminase was achieved after the optimization of selected factors.