Characterization of Polyphenols, Polysaccharides by HPLC and Their Antioxidant, Antimicrobial and Antiinflammatory Activities of Defatted Moringa (Moringa oleifera L.) Meal Extract

Alaa A. Gaafar; Eman A. Ibrahim; Mohsen S. Asker; Ahmed F. Moustafa; Zeinab A. Salama

Alaa A. Gaafar Plant Biochemistry Department, National Research Centre, P.O. Box 12622, 33-El Behouth St. Dokki-Giza-Egypt.
Eman A. Ibrahim Plant Biochemistry Department, National Research Centre, P.O. Box 12622, 33-El Behouth St. Dokki-Giza-Egypt.
Mohsen S. Asker Plant Biochemistry Department, National Research Centre, P.O. Box 12622, 33-El Behouth St. Dokki-Giza-Egypt.
Ahmed F. Moustafa Plant Biochemistry Department, National Research Centre, P.O. Box 12622, 33-El Behouth St. Dokki-Giza-Egypt.
Zeinab A. Salama Plant Biochemistry Department, National Research Centre, P.O. Box 12622, 33-El Behouth St. Dokki-Giza-Egypt.

Keywords: Phenolic, polysaccharides, antioxidant, antimicrobial, anti-inflammatory, Moringa olifera

Abstract

This study describes some active substances of hot and cold water extract from defatted cake Moringa oleifera seed. Phenolic (TP), flavonoids (TF), tannins (TT) compounds, and polysaccharide and their antioxidants, antimicrobial and anti-inflammatory activities were examined. The results showed that total phenolics of 15.76, 15.01 mg/g DW, flavonoid 3.11, 3.09 mg/g DW and tannins 4.99, 5.48 mg/g DW for cold and hot water extract respectively. According to the phytochemical assay, defatted cake Moringa oleifera showed the highest antioxidant activity, which could be related with their contents of phenolic compounds. The antimicrobial assay was examined. The results show that Moringa oleifera polysaccharide extract exhibited broad spectrum activity against the test organisms. Seven phenolic compounds (ellagic, tannic, coumarin, benzoic, catechin, and caffiec) were identified and quantified in both extracts. The polysaccharide were analyzed sugars by HPLC. Maltose, raffinose and lactose were the most abundant sugars. Profiles of 15 different natural mono sugars are identified. The results demonstrated that, defatted cake Moringa oleifera seed concentrate is an exceptionally encouraging of bioactive constituents. Therefore, it could be concluded that polyphenols present in the Moringa oleifera extract may be responsible for the antioxidant, antimicrobial and anti-inflammatory activities which could be a good source of antioxidants, antimicrobial and anti-inflammatory for food and pharmaceutical industries.