The Effect of Vitrification of Oocytes Cumulus Complex Apoptosis of Mice (Mus musculus) to Apoptosis, Rate of Fertilization and Embryo Quality

Abstract

The purpose of this study was to determine the quality of cumulus oocyte complex (COC) after vitrification process toward apotosis of COC, fertilization rate and embryo quality. Frozen occurs during the cold shock that can lead to changes in the molecular level COC. These changes will affect the quality of frozen thawed oocytes after COC. The study used two groups, There are COC not frozen and frozen COC. Parameter observed were apotosis of blastomere cells, fertilization rate and embryo quality. Apoptosis of COC were observed with tunnel apoptec staining, fertilization rates were measured based on number of zygotes and embryo quality were observed through number of blastocyst. The data of apotosis of blastomere cells were analyzed by Kruscal Wallis. The result showed that the apoptosis number, fertilization rate, and morula number between are significantly (p less then 0.05) between groups. The administration of frozen COC increase number of apoptotic blastomer cells, decreased fertilization rate up to 51.1% and embryo quality up to 69.2 %. In conclusion, Frozen on COC increased apotosis of COC, decreased both of fertilization rate and embryo quality. The embryo Frozen of vitrification is required in the ART technology necessary to add an antioxidant to improve the fertilization rate and embryo quality.