Degradation of Pesticide Chlorpyrifos by Soil Bacteria Achoromobacter xylosoxidans (Accession No. KX817809.1) with Bioremediation Potential

Abstract

Chlorpyrifos is a very widely used insecticide and considered one of the most frequently used chlorinated organophosphorus pesticides. In all the currently used method for removing such contaminants from the environment, the biodegradation has been shown to be more effective than any other method. Many pesticide degrading bacteria were isolated and identified through the colony, biochemical tests, and further identified by the 16S RNA sequencing method. The most potent strain grow in mineral salt medium (MSM) supplemented with chlorpyrifos as the sole source of carbon (150 to 1,000 μg/mL) and was monitored at an optical density of 600 nm. The growth parameters at different physical and chemical conditions were further optimized. The result showed that Achoromobacter xylosoxidans (accession no. KX817809.1) had maximum growth after 8 days. The present study shows that the isolated bacteria can be used for bioremediation of pesticide chlorpyrifos in a contaminated environment.