Designing of Multi Epitope-Based Vaccine Against SARS-CoV-2 using Reverse Vaccinology Approach

Rik . Ganguly; Angneh . Ngoruh; Prosperwell . Ingty; Atanu . Bhattacharjee

doi:10.25004/IJPSDR.2022.140415

Rik . Ganguly Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India
Angneh . Ngoruh Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India
Prosperwell . Ingty Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India
Atanu . Bhattacharjee Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India

DOI: https://doi.org/10.25004/IJPSDR.2022.140415

Keywords: Epitope,Immunoinformatics, SARS, SARS-CoV-2, vaccinology

Abstract

SARS Coronavirus-2 (SARS-CoV-2) pandemic has shown a devastating effect worldwide. More than 1.62 million people have lost their life due to this dangerous virus, which has become a major concern worldwide. It has become an emerging global issue concerning different scientific communities. Here an attempt has been made to develop a vaccine against the SARS-CoV-2 virus using epitope-based subunit vaccines and reverse vaccinology and immunoinformatics methods. With the help of computational experimentations and multi-epitope-based drug designing studies, we have successfully designed five different vaccine structures, which were further analyzed for In silico toxicity as well as molecular interaction studies. Significant interacting candidates were further subjected to In silico immuno simulation for the data point of about a year time. This work could provide new hope for the development of effective vaccine in the face of global threats arising from the emerging virus.