Assessment of Hepatoprotective Activity of Hyptis capitata Jacq. Against Oxidative Stress Induced by H2O2

V Sumitha; I Mini; Laija S Nair

V Sumitha PG and Research Department of Botany, University College, Affiliated to University of Kerala, Thiruvananthapuram, Kerala, India
I Mini PG and Research Department of Botany, University College, Affiliated to University of Kerala, Thiruvananthapuram, Kerala, India
Laija S Nair PG and Research Department of Botany, University College, Affiliated to University of Kerala, Thiruvananthapuram, Kerala, India

Keywords: Antioxidant, Hepatoprotection, HepG2 cell lines, Hyptis capitata Jacq., MTT assay.

Abstract

Medicinal plants occupy a key position in maintaining the health of the human population. Hyptis capitataJacq. is an exotic ethnomedicinal plant of Lamiaceae possessing immense potential. The present study was carried out to assess the in vitro hepatoprotective activity of methanolic leaf extract of H. capitata against H2O2 induced oxidative stress in HepG2 cell lines. Total antioxidant capacity and Superoxide radical scavenging activity of the extract were also performed. The extract displayed considerable free radical scavenging activity (P IS LESS THAN 0.01). MTT assay was used to assess the in vitro hepatoprotective activity in HepG2 cell lines. The extract displayed concentration-dependent cell viability with maximum protection (63.97 ± 1.23%) in H2O2 induced HepG2 cell lines at 100 μgml-1. H. capitata is a promising herb with significant antioxidant and hepatoprotective potential.