Hepato- and Nephroprotective Effects of the Polyphenol Concentrate From Cabernet Sauvignon Grape Varieties Cultivated in Kazakhstan in the Experimental Model Of CCL4-Induced Toxicity

Nurgozhin T., Sergazy S. H., Adilgozhina G., Gulyayev A., Shulgau Z., Abuova G., Tritek V., Saparov A.


Objective:This study investigates the hepatoprotective effect and the antioxidant role of polyphenol concentrate in the
experimental model of carbon tetrachloride (CCl4) induced toxicity. Methods: Antioxidant activity of Cabernet Sauvignon
grape polyphenol were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), 2,2’-azinobis(3-ethylbenzthiazoline-6-sulfonic
acid) (ABTS.+). In addition, the effects of polyphenol concentrate on the survival of
Wistar rats in the toxicity model, was also investigated. The polyphenol concentrate was administered for 5 five days prior
to injection of carbon tetrachloride in a sub-lethal dose of 300 mg/kg of animal body weight in order to perform histological
examinations of the liver and kidney, and detect the levels of AST, ALT and bilirubin. Results: Administration of
polyphenol concentrate increased animal survival in the experimental model. Moreover, the intragastric administration of
polyphenol concentrate prior to the initiation of the experimental model of toxicity, which was caused by a sub-lethal CCl4
dose, reduced morphological injuries in the liver and kidney, decreased the AST and ALT levels of the blood serum.
Discussion and conclusion: Our data demonstrate that polyphenol concentrate possesses an antioxidant potential both in
vitro and in vivo by reducing antioxidant stress that was caused by CCl4 administration into rats.


Free radicals; histopathology; hepatoprotective effect; antioxidants

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DOI: https://doi.org/10.25258/ijtpr.v9i03.9068


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