Development of Hepatotoxicity Model in Rats and its Application in Evaluation of Hepatoprotective Activity of Cell Wall Contents of Probiotics

Shah G. H., Patel B. G., Shah G. B.


The objective of present work is Development of Hepatotoxicity model in rats and to evaluate hepatoprotective activity
of cell wall contents of probiotics. Animals were divided in four groups. The groups were normal saline group, diseased
control group, standard drug treated group and 4th group was CCl4 +Cell wall contents of probiotics. In diseased control
group chronic liver injury was induced by administration of Carbontetrachloride (CCl4) via intraperitoneal route (1 ml/kg)
for seventy days. For standard drug treated group 1 ml Silymarin suspension (Orally) and CCl4 was given for seventy days.
In fourth group cell wall contents (1 x 10 12 CFU/ml/animal) and CCl4 was given for seventy days. During disease
induction and treatment period blood samples were collected and serum was separated which was used to analyse various
parameters like Alanine aminotransferase (ALT), Aspartate aminotransferase, (AST), Alkaline phosphate (ALP), direct
bilirubin, total protein and albumin levels to asses liver function. Along with these cholesterol, Glucose and
Malondialdehyde were also measured. Liver fibrosis and cirrhosis was quantified by histopathological studies of small
portion of the excised liver. Serum AST, ALT, ALP, and direct bilirubin were found to be significantly higher in CCl4
intoxicated rats. Total protein and albumin was decreased. Manondialdehyde was found to be significantly higher in CCl4
intoxicated rats which was main end product of Lipid Peroxidation. Whereas in cell wall contents probiotics and
silymarin treated group improve the liver functions in CCl4 toxicated rats. We conclude that protein oxidation may play a
role in the pathogenesis of CCl4 induced liver injury and that the accumulation of oxidised proteins may be an early
indication of CCl4 induced liver damage. Silymarin and cell wall contents of probiotics were effective in liver injury by
inhibiting protein oxidation.


Liver Fibrosis, Free Radicals, Lipid Peroxidation, Oxidative Stress, Carbon Tetrachloride, liver biomarkers, Cell wall content of probiotics.

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