Differences in morphological, biochemical, and genetic characteristics of grape explants grafted using various micrografting techniques

Abd EL-Zaher M.H. Sahar; M. Abd  Elwahab; Abd El-Gany   Abd El-Sattar; Heba A.    Fathy

doi:10.37855/jah.2022.v24i01.08

Abd EL-Zaher M.H. Sahar Pomology Department, Faculty of Agriculture, Cairo University, Egypt.
M. Abd Elwahab Pomology Department, Faculty of Agriculture, Cairo University, Egypt.
Abd El-Gany Abd El-Sattar Viticulture Research Department, Horticulture Research Institute, Agricultural Research Centre, Egypt.
Heba A. Fathy Viticulture Research Department, Horticulture Research Institute, Agricultural Research Centre, Egypt.

DOI: https://doi.org/10.37855/jah.2022.v24i01.08

Keywords: Grapevines, tissue culture, micro grafting, Flame seedless, Freedom, solid stacking wax and alginate matrix

Abstract

This study aimed to improve the micrografting technique for rapid in vitro grapevine plantlet propagation using Flame Seedless scion and Freedom as rootstock. Explants obtained in vitro were cultured in MS media containing 0.4 mg L-1 BAP. For micrografting, six methods were used: sterile wire ties, sterile metal needle, wax, aluminum foil, gel, and callus. Data was collected on morphological and biochemical parameters in micrografts produced using various procedures. With various degrees of success, all in vitro micrografting procedures successfully produced grafts. The best results of morphological parameters were recorded in micrografting in wax method with the highest survival (83.88%), number of shoots/explants (1.77), shoot length (1.66 cm), leaves/shoot (4.55), rooting (58.20 %), root length (1.27 cm). In contrast, the callus method had the least value for these parameters. Wax and aluminum foil methods were found to be a promising alternative to traditional grapevine propagation methods in the study.