Antimicrobial Susceptibility Profiling of Staphylococcus aureus from Bovine Clinical Mastitis Milk Samples

SUDHA  KUMARI; ANJAY .; SAVITA  KUMARI; HIMANI  SINGH

doi:10.21921/jas.v10i04.14385

SUDHA KUMARI Deptt. of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar, India
ANJAY . Deptt. of Veterinary Public Health and Epidemiology, Bihar Veterinary College, BASU, Patna, Bihar, India
SAVITA KUMARI Deptt. of Veterinary Microbiology, Bihar Veterinary College, BASU, Patna, Bihar, India
HIMANI SINGH Deptt. of Veterinary Public Health and Epidemiology, Bihar Veterinary College, BASU, Patna, Bihar, India

DOI: https://doi.org/10.21921/jas.v10i04.14385

Keywords: Bovine, clinical mastitis, Staphylococcus aureus, 16SrRNA, ABST

Abstract

Mastitis is the most common disease of bovine, leads to reduced and poor quality of milk yield causing huge economic losses. The objective of the present study was to estimate the incidence of Staphylococcus aureus in bovine clinical mastitis and determine the antibiotic susceptibility. For this purpose, a total of 71 milk samples from bovine clinical mastitis were collected from different regions of the Capital of Bihar from January 2021 to March 2022. By conventional enrichment and plating, S. aureus was isolated from 52.11% of samples. Further 39.44% incidence of S. aureus was confirmed using biochemical and molecular tools. On antibiotic susceptibility testing, the isolates were 100% resistant to penicillin and amoxicillin, while higher resistance were shown to ampicillin, oxacillin, amoxicillin/clavulanic acid, cefoxitin, erythromycin, and tetracycline.