EFFICACY OF AZADIRACHTA INDICA (NEEM) EXTRACT EMBEDDED ONTO GUIDED TISSUE REGENERATION MEMBRANE AGAINST NEISSERIA SP. CDK-10 AND MICROCOCCUS SP. CDK-23 ISOLATED FROM PERIODONTAL PLAQUE

Abstract

Periodontitis is the major oral disease after dental caries observes predominantly in developing countries like India. Severe inflammation of the periodontal ligament along with destruction of alveolar bones and teeth supporting tissue are the major complications associated with it. The central part of periodontal treatment is to restrict the progression of disease along with restoration of damaged tissues. Guided Tissue Regeneration (GTR) membrane has been shown to be a potential treatment practice for tissue restoration. However, microbial contamination on GTR membrane is a major risk associated with treatment using GTR membrane. Increasing antibiotic resistance is another major concern associated with infection control. Thus, the current study was designed to enhance treatment efficacy of GTR membrane by coating of natural antibacterial agent (neem extract) onto GTR membrane. Previously collected sample of a chronic periodontitis patient was used for the isolation of periodontal pathogens. Out of total 25 bacterial isolates, CDK-10 and CDK-23 showed highest resistance to ciprofloxacin were consider for further studies. The 16S rRNA ribotyping identifies strains CDK-10 and CDK-23 as Neisseria sp. and Micrococcus sp. respectively. Results showed that 50mg/ml Neem extract was capable to inhibit the complete growth of selected bacterial isolates. Suggesting, the sterile conditions in the periodontal pockets after phase-I therapy of Periodontitis can be maintained by coating GTR membrane with Neem extract.