WOUND HEALING ACTIVITY OF VARIOUS FRACTIONS FROM AN EXTRACT OF EHRETIA LAEVIS ROXB. (KHANDU CHAKKA) LEAVES IN ANIMAL MODEL

Abstract

Phytochemical studies are for drug discovery and very important in the discovery of new plant natural products which have commercial values and human benefits. The problem of wound treatment is getting complicated and expensive due to increasing old age population, chronic diseases, diabetes, cancer, autoimmune diseases and heavy uses of antibiotics. Ehretia Laevis Roxb. have been traditionally being used for wound care, pain management by local application in the state of Maharashtra India. An objective of this study is to know the wound repairing efficacy of various fractions of ethanolic extract of E. Laevis in an animal model. E. Laevis is also known as Ajan Vruksha and commercially known as Khandu Chakka. Ethanol extraction was done of leaves powder. n-Butanol, n-Hexane, distilled water and chloroform solvent were selected for fractionation. Weighed quantities of E. Laevis leaves fractions 5% was mixed together with simple ointment. The study of wound in animal model was done by marking the wound area on a transparent paper on 4th, 8th 12th and 16th day, using a millimeter-scale graph paper. The % of wound contraction was calculated. Control groups showed least rate of wound healing (66.77±0.28 %). Faster rate of healing was seen in groups treated with 5% w/w ointments of CFEL. WFEL 5% treated group was 68.36±0.30 %, BFEL 5% treated group had 71.86±0.30% and HFEL 5% treated groups showed 66.80±0.17 %. Reference standard heals the wound at the rate of 96.35±0.16% which is almost equivalent to the groups treated with 5% w/w ointments of CFEL i.e. 95.41±0.11.