p-NITRO BENZOIC ACID AND p-CHLORO BENZOIC ACID AS JACK BEAN UREASE INHIBITOR
Keywords:
Enzyme, Inhibition, Jack bean urease, Urea hydrolysis, Michaelis constant, Competitive inhibition.
Abstract
Enzyme inhibition is an important area of medicinal research. Enzyme inhibitor possess a wide application in agriculture, clinical science and used as drug, antibiotics and preservatives. Benzoic acid is used as preservative in many food materials. Hence inhibition of Jack bean urease catalysed hydrolysis of urea by p-nitro benzoic acid and p-chloro benzoic acid has been studied on the basis of kinetic study of hydrolysis of urea. During the experimental study the concentration of substrate was varied in the range of 1.00 x 10-2 to 2.65 x 10-2 and that of inhibitor was maintained at 2.5 x 10-3. The results of the inhibition effect are quantitatively expressed in terms of Apparent Michaelis constants Kmapp and enzymeinhibitor constant, Ki at pH 6.80 and temperature 37.0°C. Both the inhibitor shows competitive type of inhibition where both substrate and inhibitor compete with each other to react with active site of enzyme.
Published
2021-06-30
Section
Research Article
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