Standardization of isolation and genomic DNA isolation protocols of soil actinobacteria

Abstract

The concepts of ancient microbiology are promising platform for the modern microbiological aspects of identificationof isolates. The actinobacterial genera are well known for production of antimicrobial compounds under stress. Identificationand selection of actinobacterial isolates on the media will be a challenging task as the growth pattern resembles both bacterialand fungal morphology. The practical knowledge of selection and characterization is limiting due to the inability to select anisolate based on phenotypic observations. The current study is focused on selection, isolation and DNA isolation protocolof actinobacteria isolated from grass rhizosphere. The results were found convincing to select actinobacterial isolate fromthe pool of other organisms such as bacteria and fungi. The minor changes in the DNA isolation protocol resulted inimpressed DNA yield in terms of quality and quantity which can be used for sequencing purpose such that it avoidsinvesting money in DNA isolation kits and improving the basic knowledge of DNA isolation and role reagents used in eachstep for students.