Shelf life study of Pseudomonas fluorescens in liquid formulations

Abstract

Development of liquid formulations has numerous advantages which includes high cell count, prolonged shelflife and improved field efficacy. An experiment was conducted at the Institute of Organic Farming, University ofAgricultural Sciences, Dharwad to evaluate different liquid formulations of Pseudomonas fluorescens for cell viability upto twelve months of storage at room temperature. The results from shelf life studies indicated that, maximum viabilityof Pseudomonas fluorescens after twelve months of storage at room temperature was found in canola oil based formulationfollowed by soybean oil based formulation at two per cent concentration. P. fluorescens broth and talc based formulationwere not competitive in supporting the viability of Pseudomonas fluorescens. Thus, canola oil based formulation wasfound to sustain sufficient viable colonies of Pseudomonas fluorescens even after twelve months of storage period,thereby making it the promising one for storage, enhancing the shelf life and its further application in field conditionsunder organic agriculture.