Evaluation of Phytochemical Constituents and Antioxidant activity of the Roccella montagnei

Abstract

The purpose of the present study is to evaluate the phytochemical constituents of Roccella montagnei, qualitatively and quantitatively, found commonly in the Coromandel Coast, Tamil Nadu India. In the study we also executed phenolic acids identification by GCMS and more over estimation of their antioxidant potential for the very first time. As the several medicinal values have been reported previously hence this study was to find out the governing factors for these medicinal properties in Roccella montagnei. The Plant material was extracted with different solvent i.e. methanol, Hexane, chloroform, ethyl acetate and water. Phytochemical screening, Total Phenolic and Flavonoid content were analysed in all the solvent extracts the antioxidant activity was achieved by 2, 2-diphenyl-2- picrylhydrazyl (DPPH) method. Some commonly occurring phenolic were identified and quantified by GC-MS. Solvent extracts were phytochemically screened out. Total Phenolic and Flavonoid contents were estimated in different solvents extracts. Phenolic content were found in a higher amount comparative to flavonoids. Similarly comparative Antioxidant activity of various solvent extracts were determined in which methanolic extract showed marginally well antioxidant activity. Eight phenolic acids were identified by GC-MS as gallic acid, syringic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin, chlorogenic acid and 4-hydroxy benzoic acid. Overall, present study provides a suitable criterion to qualitative and quantitative evaluation of Roccella montagnei lichen sample for new vista for the exploitation of this of Roccella for the development of herbal formulations.