Biotechnological Implication with Azolla pinnata R.Br. for Metal Quenching Ability with Physiological Biomarkers.

  • Malay Kumar Adak Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani-741235, Nadia, West Bengal, India.
  • Arnab Kumar De Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani-741235, Nadia, West Bengal, India.
  • Narottam Dey Department of Biotechnology, Visva Bharati Visvavidyalaya, Santiniketan, India
Keywords: Azolla; Heavy Metals; Oxidative Stress; Antioxidative enzymes; Polyamine

Abstract

The present paper holds the accountability of an aquatic fern R.Br. for biotechnological implication. Azolla pinnata In an artificial laboratory condition Azolla plants responded well in hyper accumulation of lead in a concentration dependent manner however, within a threshold value. In accompany with metal bioaccumulation plants responded a regulated growth performances with relative growth rate (RGR) and net assimilation rate (NAR). The plants responded well with a chemical elicitors like polyamine in response to metal quenching ability. At the cellular level the phytotoxicity of the plant is mitigated by both non-enzymatic and enzymatic cascades. It is an advantage of the plants to down regulate reactive oxygen species (ROS) - superoxide (O ) and peroxide (H O ) in a moderate level as 2 2 2- compare to higher plants and modulated with polyamine. The diversity in this species was also recorded for few gene expression related to antioxidation. Fern under lead toxicity displayed well in protein polymorphism for peroxides with variations. The responses of this species for metal hyper accumulation and over expressed physiological traits are discussed in light of phytoremidiation.
Published
2016-06-30