Study on ABCG2 Gene Polymorphism in Jaffarabadi and Surti Buffaloes by PCR-RFLP

Santul Patel; Balkrishna Brahmkshtri; Umed Ramani; Guarav  Pandya; Nikhil  Dangar; Savita Devkatte; Vishnu  Kharadi

Santul Patel Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
Balkrishna Brahmkshtri Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
Umed Ramani Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
Guarav Pandya Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
Nikhil Dangar Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
Savita Devkatte Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
Vishnu Kharadi Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India

Keywords: ABCG2 gene, Jaffarabadi and Surti buffaloes, Milk production and composition trait, PCR-RFLP, Polymorphism

Abstract

In the present study, considering the role of ABCG2 gene in milk production and composition traits, Jaffarabadi and Surti buffaloes were screened for its polymorphism. PCR- RFLP technique was used to detect the polymorphism in 292 bp (partial intron 13 and exon 14) and 240 bp (partial exon 14) fragments of ABCG2 gene using HhaI and PstI restriction enzymes, respectively. The absence of polymorphism in ABCG2 gene in the study for partial intron 13 and exon 14 fragment of 292 bp as well as partial exon 14 fragment of 240 bp indicated fixation of alleles at both the loci in Jaffarabadi and Surti buffalo breeds.