Molecular Characterization of Chicken Anaemia Virus in Layers

Tejashree M. Kulkarni; Mrunalini M. Pawade; Prashant P. Mhase; Uma M. Tumlam; Vitthal . Dhaygude; Rahul P. Kolhe; Dayaram S. Suryawanshi; Dushyant M. Muglikar

doi:10.21887/ijvsbt.17.4.15

Tejashree M. Kulkarni Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India
Mrunalini M. Pawade Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India
Prashant P. Mhase Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India
Uma M. Tumlam Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India
Vitthal . Dhaygude Department of Veterinary Pathology, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India
Rahul P. Kolhe Department of Veterinary Public Health, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India
Dayaram S. Suryawanshi Director, Omega Laboratories, Lonand, Satara, Maharashtra, India.
Dushyant M. Muglikar Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirwal, Satara, Maharashtra, India

DOI: https://doi.org/10.21887/ijvsbt.17.4.15

Keywords: Chicken Anaemia Virus, Chicken embryo inoculation, Molecular detection.

Abstract

Chicken Anaemia Virus (CAV) is one of the emerging pathogens of the poultry with its genome containing three pathologically important gene viz.VP1, VP2 and VP3. In the present study, molecular detection and histopathological examination was carried out on 25 pooled tissue samples collected from birds suspected of CAV from different districts of Maharashtra. From 25 pooled samples, 20 samples were found positive for the VP3 gene with an amplicon size of 376 bp. Histopathological examination revealed the loss of stem cells in the bone marrow and lymphocytes and RBCs in the white and red pulp of spleen. Liver and kidney showed degenerative changes. The VP3 gene confirmed tissue samples were used for chicken embryo inoculation. For virus inoculation, 07 days old embryonated chicken eggs were inoculated with viral inoculum via yolk sac route. The inoculated eggs were incubated at 37?C for 10 days. The eggs were harvested after 10 days and confirmed with PCR which revealed VP3 positive results.