Effect of Season on Physico-Morphological Attributes of Cryopreserved Hariana Bull Spermatozoa

Vipin Singh, Anuj Kumar, Chhote Lal Yadav, Akhil Patel, Brajesh Yadav, Atul Saxena

Abstract


The present study was undertaken to evaluate the effect of seasonal variations on various seminal attributes and freezability of Hariana bull semen. The study was conducted over a period of four months and divided into two peak seasons as winter (December-January) and summer (May-June). Twenty-four ejaculates were collected from 4 bulls in each season. The physico-morphological characteristics of semen, viz., volume (ml), mass activity (0-5 scale), pH, sperm concentration (million/ ml), progressive motility (%), sperm livability (%), HOST (%), total morphological sperm abnormality (%) and acrosomal integrity (%) were estimated at different stages of cryopreservation in different seasons. During summer season the ejaculate volume and seminal pH were found non-significantly higher than in winter, whereas the percentage of morphologically abnormal spermatozoa was significantly (p Lass Than 0.05) higher in summer. Post- thaw percentage of progressively motile spermatozoa was significantly (p Lass Than 0.05) higher in winter than summer season. Live sperm per cent were significantly (p Lass Than 0.05) higher in winter than summer season both at fresh and pre-freeze stage. HOST positive sperm and acrosomal integrity were significantly (p Lass Than 0.01) higher in winter season than summer at all three stages. The study generated basic information that the summer season adversely affects the various physico-morphological characteristics of Hariana bull semen altering its freezability.

Keywords


Freezability, Season, Semen quality, Hariana bull.

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DOI: https://doi.org/10.21887/ijvsbt.v14i1.12997

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