https://myresearchjournals.com/index.php/ijvsbt <p><strong>The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT)is published by the&nbsp;</strong><strong>Society for Veterinary Sciences and Biotechnology (SVSBT)&nbsp;Indore (M.P.)</strong></p> <p><strong>The Indian Journal of Veterinary Sciencesand Biotechnology (IJVSBT)&nbsp;</strong>is an Open Access online and print journal that publishes Original Articles, Review Articles, Short Communications and Case Reports in all areas of Veterinary Science and its related subjects.&nbsp; Previously the journal was published as&nbsp;<strong>The Indian Journal of Field Veterinarians</strong>&nbsp;which is renamed as&nbsp;<strong>The Indian Journal of Veterinary Sciences and Biotechnology</strong>&nbsp;since 2015 and was only print version till April –June issue Vol11-4 (2016) and is converted into an Open Access online and print journal since July-Sept issue&nbsp; Vol 12-1 (2016).</p> <p><strong>The Indian Journal of Veterinary Sciencesand Biotechnology(IJVSBT)</strong>&nbsp;is a peer-reviewed, open access journal that aims to publish high quality papers rapidly and makes them freely available to researchers worldwide.&nbsp;</p> en-US Sun, 10 Jul 2022 00:00:00 +0200 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 https://myresearchjournals.com/index.php/ijvsbt/article/view/10526 . Nilima N. Brahmbhatt, Binod Kumar, Amrita A. Vasava, Vaibhavsinh D. Dodia, Bhupendrakumar J. Thakre, Avinash K. Bilwal ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10526 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10527 This study was planned to determine methane production and energy efficiency in cross bred cattle and buffaloes at maintenance on a wheat straw-based total mixed ration. The experiment was conducted in two groups consisting of seven crossbred cattle (T1) and seven buffaloes (T2) with an average body weight of 395.04 ± 44.18 and 401.43 ± 13.26 Kg, respectively. Intake of gross energy, digestible energy, and metabolizable energy (Mcal/day)of cattle (T1) was lower (p > 0.05) than buffaloes T2). Methane energy loss, when expressed daily and about percent of gross energy, digestible energy and metabolizable energy did not differ significantly. In conclusion, methane emission was non significantly (P > 0.05) lower and hence energy efficiency was higher in crossbred cattle than buffaloes on wheat straw-based total mixed maintenance ration. Pallabi Das, Bharat R. Devalia, Kalpesh K. Sorathiya, Mosam N. Chaudhary, Pravin M. Lunagariya ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10527 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10528 Azadirachta indica (neem) is an ascent, evergreen medicinal plant native to Indian subcontinent and most of the African countries. Neem causes deleterious effects on the fertility of mammals and might be used to investigate its contraceptional use in stray animal population. The aim of the study was to assess the effect of ethanolic leaf extract of A. indica on testicular morphometry (length, width, thickness and volume) and histology of male New Zealand White rabbits. Eighteen healthy, adult male rabbits, aged 12 months old, weighing between 1.83–1.90 kg were randomly divided into three groups, each group comprised of 6 animals. Group A was kept as control with distilled water only, while Group B and C were given ethanolic leaf extract of A. indica @ 100 mg/kg and 200 mg/kg body weight/day for 60 days, respectively, in drinking distilled water. Testicular length, width and thickness of both testicles were measured on day 0, 15, 30, 60 and 120 after initiation of treatments by Vernier calipers. Volume was also calculated by standard formula. The results revealed significant decrease in morphometry of the testicles at day 60 over day 0 in treated groups, and it showed variable reversal in next 60 days after discontinuation of treatment. Pushkar Sharma, Bhoopendra Singh, Sushant Srivastava, Jaswant Singh, Jitendra P. Singh, Rajesh Kumar ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10528 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10529 This study was conducted during December 2020 to June 2021 by screening 200 cows for sub-clinical mastitis by using California mastitis test and Whiteside test, and the antimicrobial efficacy of Nyctanthes arbor-tristis Linn (Jasmine, Parijatha) leaves extract was studied against mastitis pathogens. The in vitro efficacy of aqueous and methanolic extract of Nyctanthes arbor-tristis leaves and its combination was studied against a positive control, i.e., antibiotic ciprofloxacin. The isolates of Streptococcus agalactiae showed maximum zone of inhibition against combination of aqueous and methanolic extract, while S. aureus and Escherichia coli showed maximum zone of inhibition against aqueous extract. The methanolic and aqueous extracts, and its combination of Nyctanthes arbor-tristis leaves had minimum inhibitory concentration (MIC) value 50 μg/mL and 25 μg/mL, against Streptococcus agalactiae and Staphylococcus aureus, respectively. The MIC values of methanolic and combination of both extracts against Escherichia coli was 50 μg/mL in comparison to aqueous extract, i.e., 12.5 μg/mL. The aqueous extract had minimum bactericidal concentration (MBC) against Streptococcus agalactiae as 100 μg/mL. In methanolic and combination extract of N. arbor-tristis the highest value of MBC obtained for Escherichia coli, and Streptococcus agalactiae was 50 μg/mL each. Deepraj , Jitendra P. Singh, Satyavrat Singh, Dinesh K. Yadav, Ramakant , Debasish Niyogi, Rajesh K. Joshi, Dharam P. Shrivastava ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10529 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10530 This study was conducted on eighteen newborn crossbred calves (Holstein Friesian 75% × Kankrej 25%) of either sex randomly and sequentially grouped into three groups. viz. T1 (Commercial concentrate: 18% CP), T2 (Commercial concentrate: 25% CP) and T3 (Farm made concentrate: 25% CP) for period of 84 days (twelve weeks). The physiological responses of calves like rectal temperature (°F), respiration rate (no./min) and pulse rate (no./min) were recorded at weekly intervals at 07:30 am and 02:30 pm. The blood samples from individual calves were collected on 0, 42 and 84 days for hematology, serum biochemical parameters and hormone concentration. Feeding of calf starter with different level of crude protein and source has a non-significant influence on physiological responses like rectal temperature, respiration and pulse rate, hematological parameters like white blood cells, red blood cells, hemoglobin, pack cell volume, serum parameters like total protein, albumin, glucose, alkaline phosphatase, cholesterol, aspartate aminotransferase, alanine aminotransferase, triiodothyronine, thyroxine and cortisol level of crossbred calves. In conclusion, the feeding of calf starter with different sources and levels of protein non-significantly influenced physiological responses, blood parameters, and the hormonal level of crossbred calves. Harsh H. Patel, Pravin M. Lunagariya, Jigar H. Patel, Malhar R. Khant, Dilip C. Patel, Kishan N. Wadhwani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10530 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10531 In the present study, considering the role of ABCG2 gene in milk production and composition traits, Jaffarabadi and Surti buffaloes were screened for its polymorphism. PCR- RFLP technique was used to detect the polymorphism in 292 bp (partial intron 13 and exon 14) and 240 bp (partial exon 14) fragments of ABCG2 gene using HhaI and PstI restriction enzymes, respectively. The absence of polymorphism in ABCG2 gene in the study for partial intron 13 and exon 14 fragment of 292 bp as well as partial exon 14 fragment of 240 bp indicated fixation of alleles at both the loci in Jaffarabadi and Surti buffalo breeds. Santul Patel, Balkrishna Brahmkshtri, Umed Ramani, Guarav Pandya, Nikhil Dangar, Savita Devkatte, Vishnu Kharadi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10531 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10532 The continuous use of chemical ectoparasiticides leads to development of acaricide resistance in ticks. The present study was focused on evaluation of in vitro acaricidal efficacy of ethanolic extract of Azadirachta indica leaf against Rhipicephalus microplus as well as phyto analysis of A. indica leaf extract. The phytochemicals in ethanolic extract of A. indica leaf was assessed by Gas chromatography-Mass spectroscopy (GC-MS) and Fourier Transform Infra-red Spectroscopy (FTIR). The larvicidal and acaricidal effect of ethanolic extract was investigated by Adult Immersion Test (AIT) and Larval Packet Test (LPT). The FTIR analysis showed presence of carboxylic acids, alkynes, amides, aromatic esters and alkyl hallides as the functional group and GC-MS revealed presence of components; Hexadecanoic acid, octadeca-9,12,15-trienoic acid, 2-(2,5-dimethoxy-4-methylphenyl) ethanamine, 4-ethyl-1,2-dimethoxybenzene.The adult tick mortality, reproductive index, Inhibition of oviposition and larval mortality was observed 86.687%,: 0.038,: 92.59% and 78.67%, respectively at highest concentration of extract (100 mg/mL) and LC50 value was determined to be 29.21 mg/mL. There was dose-dependant mortality of adult, larva ticks and reproductive parameters. These results showed that ethanolic extract of A. indica leaves is a good source of bioactive phytochemicals with antitick activities. Manas Kumar Dalei, Manaswini Dehuri, Santwana Palai, Bijayendranath Mohanty, Senthil Kuppusamy, Ananta Hembram ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10532 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10533 Present study was carried out to assess the efficacy of aspiration and slicing methods of oocytes retrieval, retrieval rate and grades of oocytes recovered from the abattoir ovaries of culled Jaffarabadi buffaloes. The ovaries were procured from local abattoir (n=100), and the oocytes from 50 ovaries each were retrieved by slicing and aspiration method, which yielded 205 and 148 oocytes of different grades, respectively. Among the two methods of oocytes retrieval, the slicing method yielded significantly (P less than 0.01) higher average number of oocytes (4.12 ± 0.34) than the aspiration method (2.96 ± 0.29). Recovery rate of oocytes was 3.57 and 4.18 by slicing method, and 0.65 and 3.23 in aspiration method in the presence of CL and absence of CL, respectively. The mean numbers of Grade B oocytes retrieved by slicing method in the presence and absence of CL on ovary (2.00 ± 0.44, 2.19 ± 0.23) were significantly higher (p less than 0.05) than grade A (0.29 ± 0.19, 0.23 ± 0.08), grade C (0.43 ± 0.20, 0.35 ± 0.11) and grade D (0.86 ± 0.14, 1.42 ± 0.19, resp.). Similarly, the mean numbers of Grade B oocytes retrieved by aspiration method in the presence and absence of CL on ovary (0.50 ± 0.34, 1.59 ± 0.23) were higher than grade A (0.33 ± 0.21, 0.27 ± 0.07), grade C (0.17 ± 0.17, 0.25 ± 0.09) and grade D (0.00 ± 0.00, 1.11 ± 0.16, resp.). The better oocytes retrieval and good quality of oocytes were yielded by slicing method as compared to aspiration method in the absence of CL. Kiran H. Parmar, Rupesh J. Raval, Karshan B. Vala, Tapas Patbandha ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10533 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10534 The study was carried out to discern the effect of incorporating yellow mustard seed powder in diet on production performance, nutrient utilization, and egg quality of commercial laying hens. Ninety White leghorn Laying hens of 18 week were randomly distributed into three treatment groups with 30 birds per treatment with three replicates each and were subjected to feeding experiment. Hens of T1(control) group were fed with basal diet, whereas in T2 group yellow mustard seed powder @ 0.5% and in T3 group combination of mango leaf powder (0.25%) and yellow mustard seed powder (0.25%) was supplemented. Inclusion of yellow mustard seed powder in diets of layer birds significantly decreased feed intake (p ≤ 0.05), egg production (p ≤ 0.05), FCR (p ≤ 0.05) and body weight (p ≤ 0.01). Highly significant difference (p ≤ 0.01) was observed in dry matter utilization, egg weight, egg NFE while significant difference (p ≤ 0.05) was observed in feed crude protein, ether extract, NFE, glucosinolate, crude protein utilization, organic matter utilization, egg shape index, egg shell thickness, egg ether extract, egg Ca and P and egg triglyceride Diksha Uniyal, Anshu Rahal, Ashoka Kumar, Jyoti Palod, Disha Pant, Tanuj K. Ambwani ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10534 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10535 An experiment was carried out in 300 Namakkal strain of Japanese quails from day-old to five weeks of age to study the effect of supplementation of Aloe vera (Aloe barbadensis) juice at different concentrations through drinking water on growth performance. The birds were divided into four treatment groups with three replicates in each treatment and each replicate had 25 birds. The four experimental groups consisted of T0 (control), T1, T2 and T3 supplemented with Aloe vera juice @ 0, 1.0, 1.5 and 2.0%, respectively, in drinking water. The body weight of quails was measured weekly from hatch till five weeks of age and feed consumption and feed conversion ratio were calculated weekly. Mortality and livability were recorded daily and weekly, respectively. The results revealed a significant difference (p less than 0.05) in body weight and body weight gain among the groups supplemented with Aloe vera juice and control group. The cumulative feed consumption showed a significant difference (p less than 0.05) in first to fourth week, whereas on the fifth week there was no significant difference between groups. A significant difference (p less than 0.05) in feed conversion ratio among all groups supplemented with aloe vera juice and control group was observed from first to fifth week of age. Livability was not affected in quails by Aloe vera juice supplementation. Aloe vera juice supplementation (1.5%) was found beneficial in improving the overall growth performance of Japanese quails. Jogeswar P., Ponnuvel P., Sreekumar D., Mandal P.K., Ganesan R., Elanchezhiyan N. ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10535 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10536 The study was conducted on 62 adult healthy male Wistar rats (12-15 weeks of age) to examine the effect of herbal extracts on urine profile following adenine-induced chronic kidney disease (CKD). The rats were randomly divided into eight groups. Group I (n=6) was given only distilled water and served as healthy control. The rats of groups II to VIII (n=8 each) were given adenine @ 200 mg/kg body weight daily by oral gavage needle for 28 days to induce CKD. After 28th day, group II served as CKD positive group, while groups III and IV were given aqueous and alcoholic extract of Boerhavia diffusa (BD), group-V and VI with aqueous and alcoholic extract of Bryophyllum calycinum (BC) @ 300 mg/kg body weight daily for 42 days. Group VII was given bihebral aqueous extract of BC plus BD (1:1.5), while group VIII received bihebral alcoholic extract of BC and BD (1:1.5) @ 300 mg/kg body weight daily for 42 days. Urine parameters were studied on day 0, 28 and 70 of experiment to confirm the presence of CKD. Significantly (p less than 0.05) increased levels of urine output, urine specific gravity, urine calcium, phosphorus, and total protein, with decreased levels of urine creatinine and urine pH were observed in all CKD groups as compared to normal control group by 28th day. These changes were significantly (p less than0.05) reverted to normal or near normal levels within next 42 days of daily administration of either single aqueous/alcoholic extract or a combination as biherbal extract (1:1.5), without statistical differences among formulations regarding their therapeutic/nephroprotective effect against CKD in terms of reducing the altered urine values. Madhu Singh, Sunant K. Raval, Rakeshkumar J. Modi, Dashrath Sadhu, Keshank Dave ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10536 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10537 The present work was carried out on thirty Sahiwal cows selected from different villages in and around Rewa city of Madhya Pradesh. They were divided into 5 equal groups (n=6 each) to study the effect of clomiphene citrate, co-synch protocol and mineral supplementation alone or in combination on estrus induction, hormone concentration and fertility. Group 1, animals were fed ration containing 0% mineral mixture; Group 2, were fed ration containing clomiphene citrate (300 mg/ day for 5 days). In Group 3, the animals were subjected to co-synch protocol with fixed-time artificial insemination (FTAI) on day 11. Group 4 animals were fed ration containing a mineral mixture 1% and clomiphene citrate (300 mg/ day for 5 days). In Group 5, the animals were fed ration containing mineral mixture 1% and subjected to co-synch protocol. A significant difference was seen in progesterone concentration on day 42 and 60, while in estrogen, a significant difference was seen on day 9. Maximum conception rate (60%) was observed in animals that were supplemented with mineral mixture along with co-synch protocol (G5) followed by 50% with co-synch protocol alone (G3), and least conception rate (i.e., 0%) was observed in control group (G1). In group 4, mineral mixture supplementation with clomiphene citrate, no significant increase in fertility rate (33.33%) was noted. However, a higher estrus induction rate (50%) was observed, whereas in group 5 mineral mixture supplementation along with co-synch protocol increased the estrus induction (83.33%) and fertility rate (upto 60%) in anestrus Sahiwal cows. Chikkam Ajit Satya Nagendra, Jitendra Singh Rajoriya, Daniel Risheen, Snigdha Shrivastava, Vedha Vyas Reddy ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10537 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10538 An attempt was made to predict standard lactation milk yield (SLMY) by simple regression equation from weekly, fortnightly and monthly, individual and cumulative part yield in Jaffarabadi buffalo. A total of 176 normal lactations of Jaffarabadi buffaloes from 1st to 6th parity with lactation length >210 days were analysed. Correlation coefficients between 305-days milk yield (SLMY) and different weekly (1st to 20th week), fortnightly (1st to 5th fortnight) and monthly (1st to 5th month), individual and cumulative part lactations were positive and significant (P less than 0.01) and showed increasing trend with advancement of lactation. The reliability of prediction for SLMY was reasonably high, i.e., 63 to 67% for 18th or 19th week, 67 to 68% with 9th or 10th fortnight, and 63 to 67% for 4th or 5th month part lactation yields when used as independent variables. From 20th week, the correlation and accuracy of regression for SLMY reduced. Individual part yields were found to be more reliable than cumulative respective (weekly, fortnightly and monthly) part yields for prediction of SLMY. First 100-days milk yield as part lactation covered only 49% of variation in SLMY in Jaffarabadi buffaloes, which implies that one must be cautious while sire evaluation based on first 100-d yield as part lactation of daughters under progeny testing programmes or determining production potential in terms of LMY based on part lactation of Jaffarabadi buffaloes. Pravin N. Chaudhari, Mahesh R. Gadariya, Pratik S. Kapadiya, Pranav M. Gamit, Bhagavanji D. Savaliya ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10538 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10539 This study was conducted on 13 bulls of Kankrej cattle breed maintained at Dama semen station of Banas dairy (Gujarat) to study the effects of non-genetic factors, viz., season of birth and year of birth on body weight at first semen collection (BWFSC) and scrotal circumference at first semen collection (SCFSC) as well as the effect of BWFSC and SCFSC on future semen production traits like semen production period, age of bull at disposal and lifetime production of frozen semen doses per bull. The overall least squares means for body weight of bulls and scrotal circumference of bulls at first semen collection, semen production period, age of bull at disposal and lifetime production of frozen semen doses per bull were 578.68 ± 25.18 kg, 30.86 ± 1.60 cm, 704.3 ± 151.22 days, 1875.73 ± 135.95 days and 55243.83 ± 21307.60 doses, respectively. Results of the present study revealed that body weight and scrotal circumference of bulls at first semen collection were not significantly affected by season of birth and year of birth. Similarly, BWFSC and SCFSC had no significant effect on semen production traits. Nikhil S. Dangar, Balkrishna P. Brahmkshtri, Gaurav M. Pandya, Niteen Deshmukh ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10539 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10540 The ability to predict the frozen semen doses produced per ejaculate would be of considerable benefit for the management of skill, human resource, capital and time. The new computing paradigm called machine learning involves in predicting dependent variable by learning complex and non-linear relationship among independent variables. The purpose of this study is to develop prediction model using one of the conventional and machine learning modelling techniques called Multiple Linear Regression (MLR) and Artificial Neural Network (ANN), respectively. A Total of 1,57,532 ejaculates data were used for modelling. The modelling involved prediction of frozen semen doses produced per ejaculate using independent variables namely volume of ejaculate, ejaculate number, sperm concentration, initial motility and post thaw motility. Various combinations of architectural parameters were employed to explore optimum configuration for each model. The ANN (R2=90.66) modelling was observed to be efficient over MLR (R2=73.52). The root mean squared error (RMSE) value was found to be lower in ANN (33.89) when compared to MLR (57.31). Hence, the ANN modelling approach is efficient to predict frozen semen doses that could be produced per ejaculate Chandrashekharaiah Jeevan, Sarojini M.K. Karthickeyan, Alagappan Gopinathan, Gopalan K. Tirumurugaan, Subbiah Vairamuthu ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10540 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10541 Pig farming is gaining momentum as a promising source of unconventional livestock farming in the current global scenario in which the food habits of Indian consumers is changing. It is one of the untapped livestock sectors, attracting entrepreneurs from all dimensions, searching for a great profit-generating source of livelihood, fetching sustainable returns if managed on scientific lines. Under the RKVY-RAFTAAR scheme of the Government of India, the Agribusiness Incubation Center (ABIC) of ICAR-Indian Veterinary Research Institute (IVRI), also called Pashu-Vigyan Incubator is continuously organizing the piggery Entrepreneurship Development Programme (EDP) to popularize pig farming in India. The present paper, based on ex-post-facto research conducted at IVRI enlightens the atmosphere around scientific pig farming. It ascertains the attitude of the trainees towards scientific pig farming and the EDP as a whole. A purposive sampling technique selected 80 trainees who positively started their piggery enterprises after attending the EDPs at Pashu-Vigyan Incubator IVRI Izatnagar. The data collection was done through e-questionnaires and telephonic follow-up. Majority of the trainees agreed that the ‘government should organize training for scientific piggery management’, believed that ‘cross bred animal is a symbol of progressiveness’ and ‘looked forward to adopting scientific piggery practices’. Almost half of them held ‘favourable’ attitude towards piggery entrepreneurship, followed by 42% who held ‘more favourable’ attitude towards the same. Akriti Anna, Braj Pal Singh, Mahesh Chander ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10541 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10542 A study was conducted on 18 dogs suffering from demodectic mange brought to the Veterinary Clinical Complex (VCC) of the College in Mhow and at Private Clinics, Indore. The dogs were randomly subdivided into three groups having 6 dogs in each group. Group I dogs were treated with Inj. Ivermectin 1% S/C + solution Amitraz 12.5% @ 5 mL/L applied topically once a week for 6 weeks. Group II dogs were treated with spot-on preparation of Moxidectin 2.5% + Imidacloprid 10% applied topically once a week for 6 weeks, while Group III dogs were treated with topical application of herbal preparation containing extract of Azadirachta indica (Neem) leaves + Aloe vera gel daily twice for six weeks. The treatment response of animals was evaluated by improvement in clinical signs and haemato-biochemical parameters. No side effects were reported in any group. The results showed significant improvement in haemato-biochemical parameters. Combination of Inj. Ivermectin 1% S/C + solution Amitraz 12.5% was found to be superior to the herbal and spot-on preparations. Naman Gupta, Rajendra K. Bagherwal, Hemant K. Mehta, Vivek Agrawal, Ranjit Aich ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10542 Sun, 10 Jul 2022 00:00:00 +0200 https://myresearchjournals.com/index.php/ijvsbt/article/view/10543 The present study was focused on comparing lateral flow assay (LFA) and one-step RT-PCR for detection of Canine distemper virus (CDV) in dogs. Total of 74 swab (38 nasal and 36 conjunctival) samples from 23 CD suspected dogs were collected. Detection of CDV was performed by LFA using quickVET Rapid test kit and molecular detection by one-step RT-PCR targeting N gene. Based on the test result, sensitivity and specificity were calculated. The prevalence of CD detected by LFA and RT-PCR were 69.56% and 73.91% respectively among dogs.. All the dogs positive for CDV by LFA were also positive by RT-PCR, including one more dog. Comparative analysis of RT-PCR and LFA using sample-wise positivity revealed that relative sensitivity and specificity of LFA considering RT-PCR as the Gold standard test were 66.66% and 100%, while overall agreement between the two assays was 77.03%. For diagnosis of CD in dogs, RT-PCR proved to be a better test than LFA. However, the inherent advantages associated with field tests like LFA and its considerable sensitivity and specificity can be useful and adopted for field-level diagnosis. Vedanshee R. Joshi, Mayurdhvaj K. Jhala, Bharat B. Bhanderi, Vipul R. Nimavat, Dhruv N. Desai ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-nd/4.0 https://myresearchjournals.com/index.php/ijvsbt/article/view/10543 Sun, 10 Jul 2022 00:00:00 +0200