Lipase production by three thermophilic fungi
Keywords:
Thermophilic fungi, lipase, influencing factors, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus
Abstract
Production of lipases by three thermophilic fungi, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus was investigated, influence of substratum, pH, temperature, carbon and nitrogen sources on lipase production was also studied. All the three fungi under study produced good amount of lipase constitutively. Lipase production was maximum at 45oC and it was thermostable.
References
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2. Rajan, A., Sudha, J.D. and Abraham, T.E., 2008, “Enzymatic modification of cassava starch by fungal lipase”, Indust. Crops and Prod., 27, pp. 50-59.
3. Gasper, M.I., Cummigham, M. and Polleri, P., 1999, “Occurrence and properties of extracelluar lipase in Mortierella vinacea”, Mycologia., 91, pp. 108-113.
4. Lima, V.M.G., Krieger, N., Sarquis, M.I.M., David, A., Mitchell, D.A., Ramos L.P. and Fontana, J.D., 2003, “Effect of nitrogen and carbon sources on lipase production by Penicillium aurantiogriseum”, Food Technol. and Biotech., 41, pp.105-110.
5. Crooks, G.E., Rees, G.D., Robinson, B.H., Stvensson, M. and Stephenson, G.R., 1995, “Comparison of hydrolysis and esterification behavior of Humicola lanuginosa and Rhizomucor miehei effect of temperature on reaction kinetics and general considerations of stability and productivity”, Biotech. and Bioeng., 48, pp.190-196.
6. Gandhi, N.N., Vijayalaksmi, V., Sawant, S.B., and Joshi, J.B., 1996, “Immobilization of Mucor meihei lipase on ion exchange resins”, Chem. Engin. J, 61, pp.149-156.
7. Dellamore, O.G.M., Martinus, R.G., Roche, W.L. and Dias, A.P., 1997, “Activity and stability of a Rhizomucor lipase in hydrophobic media”, Biotechnol. Appl. Biochem., 26, pp.31-37.
8. Cooney, D.C. and Emerson, R., 1964, “Thermophilic fungi: An account of their Biology, Activities and Classification”, San Fransisco; London, W.H. Freeman& Co., pp-188.
9. Bornscheuer, U.T., Bessler, C., Srinivas, R. and Krishna, S.H., 2002, “Optimizing lipases and related enzymes for efficient application”, Trends Biotechnol., 20, pp. 433-437.
10. Urs, K.M., G.S.Bains and Bhatia, D.S., 1962, “Triacetin as substrate for peanut lipase”, Sci and Cult., 28, pp.581-582.
11. Aulakh, S.S. and Prakash, R., 2010, “Optimization of medium and process parameters for the production of lipase from an oil-tolerant Aspergillus sp. (RBD-01)”, J. Basic Microbiol., 50, pp. 37–42.
12. Ginalska, G., Cho, H.Y., Cho, N.S., Bancerz, R., Kornil, O.T., Leonowicz, A., Shin, S.J. and Ohga, S., 2007, “Effect of culture conditions on growth and lipase production by a newly isolated strain, Geotrichum-like R59 (Basidiomycetes)”, J. Fac. Agr. Kyushu. Univ., 52, pp.29–34.
13. Savitha, J., Srividya, S., Jagat, R., Payal, P., Priyanki, S., Rashmi, G.W., Roshini, K.T. and Shantala. Y.M., 2007, “Identification of potential fungal strains for the production of inducible, extracellular and alkalophilic lipase”, Afr. J. Biotech., 6, pp. 564-568.
14. Abbas, H., Hiol, A., Deyris, V. and Comeau, L., 2002, “Isolation and characterization of an extracellular lipase from Mucor spp.strain isolated from palm fruit”, Enzy. Microb. Technol., 31, pp. 968-975.
15. Mhetras, N.C., Bastawde, K.B. and Gokhale, D.V., 2009, “Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207”. Biores. Technol., 100, pp.1486-1490.
Published
2023-09-13
Section
Articles