Embelin - An HPLC Method for Quantitative Estimation in Embelia ribes Burm. F.

Abstract

An RP-HPLC method with photodiode array detection was established for the determination of major constituent, Embelin in Embelia ribes samples. The Embelin was separated by using isocratic mode consisting of 0.1 % trifluoroacetic acid in water and methanol (in proportion of 88:12) at a flow rate of 1.0 mL/min. Under these conditions, a plot of integrated peak area versus concentration of Embelin was found to be linear over the range of 5.0-75.0 N less than g/mL, with a relative standard deviation of 0.61-0.96 %. The limit of detection was 20 ng on column and the limit of quantitation was 50 ng on column. The determination of the Embelin content in various solvent extracts exhibited a mean content of 0.44-33.0 % w / w. Recovery experiments led to a mean recovery rate of 96.49 B1 2.42 %. The proposed method is less time-consuming, sensitive and reproducible and is therefore suitable for routine analysis of Embelin in various extracts of E. ribes.