Evaluation of Flower Head Alcoholic Extract of Sphaeranthus indicus Linn. On Wound Healing in Diabetic Albino Rats

Abstract

The basic objective of the present work was to assess the wound healing activity of alcoholic extract of Sphaeranthus indicus flower head by providing better tissue formation and protection against microbial invasion. The flower heads of Sphaeranthus indicus were subjected for extraction with ethanol. Various ointments of extracts in various proportions were prepared and subjected for assessment of wound healing activity using four parameters i.e., Wound Contraction Studies (Excision Wound), Tensile Strength Measurement (Incision Wound), Hydroxyproline Content Determination (Incision Wound) and Histopathological Studies (Incision Wound). Based on the comparison of wound healing activity of various formulations, the formulation comprising of 2 % (w/w) alcoholic extract found to be superior to that of control and standard formulation (0.3 % w/w Neomycin standard ointment). The present studies evidenced the significant wound healing activity of Sphaeranthus indicus by increasing cellular proliferation, formation of granulation tissue, synthesis of collagen and by increase in the rate of wound contraction.