Antibacterial effectiveness of many plants extracts against the resistant Negative Coagulase Staphylococcus that cause Clinical Mastitis in Cows

Abstract

Clinical mastitis caused by negative coagulase staphylococcus is considered to be the most dangerous diseased cases in cows and it can cause great economic loses. The aim of this study was to investigate the effectiveness of extracts prepared from different parts of the following plants: Olea europea Linn (Oleaceae) , Myrtus communis Linn (Myrtaceae) , thymus vulgaris Linn (Laminaceae), Rosmarinuis officinalis Linn (Laminaceae) Ficus carica Linn (Moraceae),and Achillea falcata Linn (Asteraceae), against resistant, negative coagulase staphylococcus in 1371 Samples of milk. The presence of negative coagulase staphylococcus in 1371 Samples of milk by using blood agar, Manitol agar, and some bio-chemical tests that were investigated .Secondly, the antibacterial activity of many antibiotics on these bacteria by using disc diffusion method were determined . The plants were extracted with water, absolute alcohol, then ether by using soxhlet apparatus and rotary vacuum evaporator. The antibacterial activity of the plants extracts were determined by using disc diffusion method. This study showed the presence of different antibacterial effectiveness of the alcoholic extracts prepared from different parts of those plants against resistant coagulase staphylococcus. The extract of thymus vulgaris has the strongest effectiveness, whereas the extract of Olea europea has the weakest effectiveness against resistant coagulase staphylococcus. The alcoholic extracts of the studied plants showed the antibacterial effectiveness against resistant coagulase staphylococcus.