Evaluation of Anticancer Activity of Pinus roxburghii Sarg. Against IMR-32 Human Neuroblastoma Cancer Cell Line.

Abstract

To study the anticancer activity of petroleum ether, ethyl acetate, chloroform and ethanol extract of Pinus roxburgii Sarg. by SRB assay method on IMR-32 Human Neuroblastoma cancer cell line. Anticancer activity of different extracts of Pinus roxburgii Sarg. was performed on IMR-32 Human Neuroblastoma cancer cell line by the Advanced Centre for Treatment Research and Education in Cancer (ACTREC) Mumbai, India. Out of the four extracts petroleum ether and chloroform extract have showed anticancer activity on IMR-32 Human Neuroblastoma cancer cell line. Cell line were grown in RPMI 1640 medium containing 10% fetal bovine serum and 2mM L-glutamine. Present study implicit the observation that petroleum ether and chloroform extract of the plant Pinus roxburghii Sarg. showed a promising anti-cancer activity against only one cell line and may be active against the other cell lines. Further, all these plants extract need to be screened against different cell lines apart from the selected cell line to confirm the activity.