Antihyperglycemic Activity of Edible Mushroom, Lentinus edodes in Alloxan Induced Diabetic Swiss Albino Mice

Abstract

Consequences a cure for diseases and infections using herbal medicine are primordial approach. The present study aimed at investigation of anti-hyperglycemic activity of 50% ethanolic extract of edible mushroom, Lentinus edodes, in alloxan induced diabetic Swiss Albino Mice (SAM). There are a few unequivocal studies to authorize in-vitro anti-diabetic activity of edible mushroom. This prospective study was designed to comprehend the hypoglycemic properties of edible mushroom on the diabetes mellitus. Seven groups of SAM consisting of each group with five male SAM were used in this experiment and diabetes in each SAM was induced by intraperitoneal injection of 180 mg/kg alloxan excepting group-VII (G_VII). The group-I (G_I) served as a negative control and received a daily intraperitoneal injection of normal saline solution (1 ml / 100g of 0.9 % NaCl, V/W), and group-II (G_II) also assisted as positive control receiving a daily intraperitoneal injection of metformin (3.33 mg/Kg), and the subsequent groups of SAM namely group-III(G_III), group-IV(G_IV), group-V(G_V), and group-VI (G_VI) were received mushroom extract/daily by using intragastric feeding tube. The ethanolic extract of Lentinus edodes at the dose of 200, 400, 600 and 800 mg /kg body weight was administered orally once a day to the groups of G_II, G_IV, G_V, and G_VI for 3 days and then the fasting blood glucose was estimated in both normal and alloxan induced diabetic SAM. The fasting blood glucose was found to be significantly reduced (p less than 0.05) in treated SAM in compared to control SAM groups. The study discloses that Lentinus edodes has significant antidiabetic activity in alloxan induced diabetic SAM. So, The Lentinus edodes extract appears promising for the development of a phytomedicine for diabetes mellitus.