A Rapid Bio-Analytical Method for Simultaneous Quantification of Boeravinone B and Eupalitin-3-O-Β-D-Galactopyranoside from Boerhaavia Diffusa Using LC-MS/MS

Abstract

Today people are more dependent on herbal drugs with over 80 percent of the world population relying on the traditional systems of medicine, largely plant based, for treatment of several acute diseases. The current research is based on the plant Boerhaavia diffusa Linn. which has been widely used for treating acute diseases and possess various biological activities. An array of pharmacodynamic studies have been conducted on animals using this plant. Inorder to bridge the gap between pharmacokinetics and pharmacodynamics, an understanding of the pharmacokinetic profile of the active markers in the plant is necessary for improved drug therapy. In the current research a rapid and sensitive Bio-analytical LC–MS/MS method has been developed and validated for simultaneous quantification of active markers Boeravinone B and Eupalitin-3-O-β-D-galactopyranoside in human plasma. The analytes were extracted from human plasma by SPE. Sildenafil citrate was used as the internal standard. A RP18 column enabled chromatographic separation of the analytes. The method involves simple isocratic chromatography and MS detection in positive-ionization mode. Validation of the method showed response was a linear function of concentration in the range 5.0–500.0 ng mL−1 for both Boeravinone B and Eupalitin-3-O-β-D-galactopyranoside. The method was suitably validated and was found to be precise and robust, with recoveries for both the analytes being consistent. The method can be successfully applied for the analysis of actual samples from dosed human and animals from pharmacokinetic studies.